Journal:
Article Title: Gene expression profiling of TGF?2- and/or BMP7-treated trabecular meshwork cells: Identification of Smad7 as a critical inhibitor of TGF-?2 signaling
doi: 10.1016/j.exer.2009.01.002
Figure Lengend Snippet: Antibodies used for Western blot and immunohistochemistry in the present study (WB, Western blot; IHC, immunohistochemistry)
Article Snippet: Exposure times ranged between 1 and 5 min. Quantification was done using the AIDA Biopackage software (Raytest, Straubenhardt, Germany). table ft1 table-wrap mode="anchored" t5 caption a7 Antibodies Abbreviation Dilution Companies fluorescein swine anti goat IgG Sw anti G-Cy3 1:100 Dako, Glostrup, Denmark horseradish peroxidase-conjugated chicken anti goat IgG Ch anti G-HRP 1:2000 Santa Cruz Biotechnology, Santa Cruz, USA horseradish peroxidase-conjugated chicken anti rabbit IgG Ch anti R-HRP 1:2000 Santa Cruz Biotechnology, Santa Cruz, USA polyclonal goat anti human CTGF G anti hCTGF 1:500 (WB) Santa Cruz Biotechnology, Santa Cruz, USA polyclonal rabbit anti human SMAD7 rb anti hSMAD7 1:700 (WB) Santa Cruz Biotechnology, USA Polyclonal rabbit anti human VEGF Rb anti hVEGF 1:500 (WB) Santa Cruz Biotechnology, USA Polyclonal goat anti human TFPI G anti hTFPI 1:1000 (WB) 1:200 (IHC) Santa Cruz Biotechnology, USA Polyclonal goat anti human MRCKβ G anti hMRCKβ 1:300 (WB) Santa Cruz Biotechnology, USA Open in a separate window Antibodies used for Western blot and immunohistochemistry in the present study (WB, Western blot; IHC, immunohistochemistry) Immunohistochemistry HTM cells were grown on microscopic slides to preconfluency and treated as described above.
Techniques: Western Blot, Immunohistochemistry